Studies on N-Ethyl-N-nitrosourea Mutagenesis in BALB/c Mice
نویسندگان
چکیده
منابع مشابه
N-ethyl-N-nitrosourea mutagenesis: boarding the mouse mutant express.
In the mouse, random mutagenesis with N-ethyl-N-nitrosourea (ENU) has been used since the 1970s in forward mutagenesis screens. However, only in the last decade has ENU mutagenesis been harnessed to generate a myriad of new mouse mutations in large-scale genetic screens and focused, smaller efforts. The development of additional genetic tools, such as balancer chromosomes, refinements in geneti...
متن کاملInduced by N-ethyl-N-nitrosourea
Phenotypic reversion of the rubber-band, muscledefective phenotype conferred by unc-93(e1500) was used to determine the utility of Nethyl-Nnitrosourea (ENU) as a mutagen for genetic research in Cuenmhabditis ekguns. In this system, ENU produces revertants at a frequency of 3 X equivalent to that of the commonly used mutagen, EMS. The gene identity of 154 ENU-induced revertants shows that the di...
متن کاملAn N-ethyl-N-nitrosourea mutagenesis screen for epigenetic mutations in the mouse.
The mammalian epigenetic phenomena of X inactivation and genomic imprinting are incompletely understood. X inactivation equalizes X-linked expression between males and females by silencing genes on one X chromosome during female embryogenesis. Genomic imprinting functionally distinguishes the parental genomes, resulting in parent-specific monoallelic expression of particular genes. N-ethyl-N-ni...
متن کاملA monogenic dominant mutation in Rom1 generated by N-ethyl-N-nitrosourea mutagenesis causes retinal degeneration in mice
PURPOSE To characterize an N-ethyl-N-nitrosourea-induced dominant mouse mutant, M-1156, that exhibits progressive retinal degeneration and to investigate the pathogenesis of the retinal phenotype in the mutant. METHODS A positional candidate gene approach was used to identify the causative gene in the M-1156 mutant. Funduscopic examination, light microscopy, transmission electron microscopy, ...
متن کاملIdentification of a novel point mutation of mouse Atp2b2 induced by N-ethyl-N-nitrosourea mutagenesis.
N-ethyl-N-nitrosourea (ENU)-induced mutagenesis is an important approach in the study of gene function and the establishment of human disease models. Here we report an ENU-induced mutation, Elfin, as a mouse model with hearing loss. Homozygous mutants were deaf and displayed severe ataxia, while heterozygous mice had a significant hearing loss. Histological analysis of the inner ear revealed th...
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ژورنال
عنوان ژورنال: Toxicological Research
سال: 2008
ISSN: 1976-8257
DOI: 10.5487/tr.2008.24.1.059